Bottled (500ml) serum thawing step (gradual thawing method): -20 ° C or -70 ° C to 4 ° C refrigerator dissolved in one day, completely dissolved at room temperature and then repacked, generally 50 ml sterile centrifuge tube can be divided into 40 ~ 45 ml. In the process of dissolving, it should be shaken regularly (be careful not to cause bubbles), so that the temperature and composition are uniform, and the precipitation is reduced. Do not directly thaw from –20 °C to 37 °C. Because the temperature changes too much, it is easy to cause protein to coagulate and precipitate.
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