Hengyuan technicians analyze Elisa kit washing according to their own experience - Database & Sql Blog Articles

Recently, many customers have inquired about the problem of washing the elisa kit. Many customers have made mistakes in the elisa kit washing step, which has an impact on the experimental results. Shanghai Hengyuan technicians analyze Elisa washing based on their many years of experience and share them with friends here! I hope to help you with your experiment. The washing step reduces the background signal caused by unbound antibodies, thereby increasing the signal to noise ratio of the assay. Washing between each step ensures that only specific binding events are retained and signals are generated in the final step. Inadequate washing can lead to differences and high backgrounds, resulting in undesirable results. This article will introduce you to some tips for using the automatic washer to wash Elisa tablets. The second major parameter affecting the washing effect is the amount of washing cycle. Of course, the more the number of washings, the lower the background. However, too many washes reduce the signal strength, making it difficult to measure. It is common practice to repeat the wash three times after each incubation of the antibody or antigen. However, manufacturers of Elisa boards advise on the number of washes. In general, the manufacturer's packaged tablet requires fewer washes than the user's packaged tablet. For Elisa boards that are covered by the user, the number of washes must be optimized. Another method of controlling the amount of washing and the number of washings is to add an excess of washing liquid. In general, each well of a 96-well plate can hold 330 to 460 μl. However, some automated washers can be programmed to dispense a wash solution that is well above this amount, such as 1 ml. How is it done? In fact, it is very simple, that is, the liquid absorption function is turned on at the same time as the liquid separation. In other words, as the dispenser dispenses more liquid, the aspirator also draws liquid out. This technique increases the amount of washing but does not spill into other holes. Do you still have a clear understanding of the above Elisa kit washing experience analysis? Shanghai Hengyuan Bio-Elisa Kit provides free testing and full-service technical support services. For more information about Elisa technology, please feel free to call our sales staff.