Solution to intracellular cytokine flow detection (Shanghai Jinma Biotechnology Co., Ltd.)
How important is BD Fc BlockTM reagent when performing intracellular factor staining? How to apply it?
In flow cytometry, we found that the use of Fc receptors is essential, especially for monocytes or granulocytes. The BD FcBlock should be used before the rupture of the membrane. BD currently offers BD Fc Block products for mice and rats. For intracellular staining of human cells, blockade of Fc receptors is not particularly important. In the case of detecting a human sample in a specific case, a conventional blocking agent such as an irrelevant purified Ig of the same species, or a serum of an antibody-derived species may be applied. This will reflect the importance of the reagents. Thank you for your support of Shanghai Jinma Bio. I wish you all the best!
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