Monoclonal antibody service

Shanghai Jinma Experimental Equipment Co., Ltd. specializes in antibody production and customization services, and has its own rich and unique experience in the development of mouse monoclonal antibodies. In recent years, we have continuously optimized the monoclonal antibody experimental system. From the immunization, hybridoma cell fusion to the screening of positive hybridoma cell lines, we have been in-depth improvement, greatly improving the fusion rate and positive rate. In the development of murine monoclonal antibodies, whether you provide antigens or we prepare small molecules, peptides or protein antigens based on the antigen information you provide, we will provide you with the highest quality monoclonal antibodies in the shortest possible time. . For mouse monoclonal antibodies that are difficult to prepare, we have sufficient experience and expertise to clone the murine antibody gene and prepare active and functional genetically engineered antibodies using various protein expression platforms. At the same time, in the purification of monoclonal antibody antibodies, we also have a professional technology to purify mouse antibody types including IgG1, IgG2a, IgG2b and IgM.

With our professional and comprehensive R&D team, whether your monoclonal antibody is used for scientific research, for the development of diagnostic reagents, or for the preliminary research of antibody drugs, we will combine our research and development for many years. Experience Through good communication with you and our in-depth analysis of the project itself, we hope to ensure the best possible monoclonal antibody for your experiment from the early stage of the design of the experiment.

Finally, provide: 2-3 hybridoma cell lines and 10 ml of ascites purified antibody, 100 ul of ascites stock solution, 1 ml of cell supernatant, and give the corresponding identification report (including the following: mouse serum titer, hybridoma construction) (fusion rate, positive rate), cloning of hybridomas, antibody-specific detection of hybridoma culture supernatants, and ELISA titer detection of hybridoma-inducing ascites).

The final guarantee: the supernatant of the provided monoclonal antibody ascites or hybridoma cell line is specifically bound by the ELISA test and the antigen sample provided by the customer; the supernatant of the provided monoclonal antibody ascites or hybridoma cell line is detected by Western blot. Positive.

2, monoclonal antibody customized service type

CSBYF03A1

Mouse monoclonal antibody preparation service (customer provided antigen)

4-6 months

Inquiry

CSBYF03A2

Mouse monoclonal antibody preparation service (Jinma provides antigen)

5-7 months

Inquiry

CSBYF03A3

Rat monoclonal antibody preparation service (customer provided antigen)

4-6 months

Inquiry

CSBYF03A4

Rat monoclonal antibody preparation service (Jinma provides antigen)

5-7 months

Inquiry

CSBYF03B1

Mouse Rapid Monoclonal Antibody Preparation Service (Customer Provided Antigen)

8-10 weeks

Inquiry

CSBYF03B2

Mouse Rapid Monoclonal Antibody Preparation Service (Jinma Provides Antigen)

9-14 weeks

Inquiry

CSBYF03B3

Rapid monoclonal antibody preparation service in rats (customer supplied antigen)

12-14 weeks

Inquiry

CSBYF03B4

Rapid monoclonal antibody preparation service in rats (Jinma provides antigen)

14-18 weeks

Inquiry

CSBYF03C

Anti-idiotype monoclonal antibody preparation service (mouse)

advisory

Inquiry

CSBYF03D

Modified monoclonal antibody preparation service (Jinma provides antigen)

advisory

Inquiry

CSBYF03E

Preparation of monoclonal antibodies in serum-free medium

4-6 months

Inquiry

Service number	service name	Service cycle	service fee

3. Technical advantages

1 latest service package: antibody preparation, antibody purification, antibody screening integration services; can provide a variety of purification methods, ammonium citrate, Protein A / G, antigen affinity chromatography; antibody screening saves you time, we can According to customer needs, screening of antibody pairs and natural sample identification verification, so that customers have no worries.

2 Diversified Customized Service: It can provide mouse and rat monoclonal antibody service platform, rapid preparation of monoclonal antibody service platform in semi-solid mode, and serum-free medium monoclonal antibody preparation service platform. Customers can flexibly choose according to their needs.

3 Antigen and immune species diversity: Jinma has a powerful protein service platform. Customers can choose the antigen to provide antigen. There are many expression systems for you to choose. For example, breast milk protein is closer to the natural conformation, which can make your antibody higher quality. In addition, we have immunized at least 5 mice/rats per project, using multiple immunization routes, and multi-pronged approach to provide a better quality monoclonal antibody.

4 large-scale production services: can provide the most professional large-scale production of monoclonal antibodies to meet the needs of diagnostic-level mass production.

5 High quality: specializing in antibody production and customized services, a series of related technology platforms and animal breeding bases have been established with the antibody platform as the core. The monoclonal antibody products we provide are completed by professionals who are engaged in antibody production all the year round. The technical route is mature, the product quality is stable and reliable, and the sensitivity is extremely high. So far, the platform has successfully prepared a variety of diagnostic reagents for monoclonal antibody to raw materials. Praise from downstream customers.

Affordable: Monoclonal antibodies are highly specific and sensitive, and our services are competitively priced to provide you with high quality products while saving costs.

CSBYF0301

Animal immunity and ELISA

9-12 weeks

Â¥**.00

CSBYF0302

Fusion and ELISA screening

1 week

Â¥**.00

CSBYF0303

Subcloning and ELISA screening

4-6 weeks

Â¥**.00

CSBYF0304

Cell expansion culture and ELISA detection

2 weeks

Â¥**.00

CSBYF0305

Subtype identification (1 strain)

1 week

Â¥**.00

CSBYF0306

Preparation of ascites (1 strain)

2 weeks

Â¥**.00

CSBYF0307

Ascites purification (1 strain)

1 week

Â¥**.00

CSBYF0308

Epitope identification

1 week

Â¥**.00

Service number	Project Description	Service cycle	unit price

Three. Service instance

Working closely with many well-known scientific research reagent companies, diagnostic reagent companies, and biopharmaceutical companies around the world, we have continuously developed monoclonal antibodies that meet the specific application requirements of our customers, such as those used in ELISA, IHC, IP, western blot and other technology platforms. Antibodies; can develop a pair of monoclonal antibodies suitable for their specific application requirements, enabling them to be applied to diagnostic antibodies on ELISA, colloidal gold test strips, chemiluminescent reagents or immunomagnetic beads; or developed for customers High sensitivity small molecule, peptide monoclonal antibody; professional, efficient and economical is our solemn commitment to customers.

Brand authorization genuine real shot worry-free return thousands of trustworthy customer service lightning delivery preferred benefits

1. Diagnostic grade monoclonal antibody customization

1 A monoclonal antibody developed by our company for the screening of 4 different epitopes can be paired in pairs; and successfully applied to the development of ELISA, colloidal gold, latex immunoenhanced nephelometric kit

Figure 1.1. Stability test of a monoclonal antibody-resistant immunoturbidimetric assay

(linear and

Good stability)

Figure 1.2. Clinical comparison of a monoclonal antibody-enhanced immunoturbidimetric assay

2 Our company develops a monoclonal antibody pair for customers, and can identify natural samples by WB, ELISA, IHC and other methods.

Figure 1.3. A monoclonal antibody against a Western Blot

All lanes

: monoclonal antibody at 1μg/ml

Lane 1:

HepG-2 cell lysate

Lane 1:

Transfected pichia Yeast cell lysate

Predicted band size :

23 kDa
Observed band size :

23kDa

Additional band size: 50KD

Figure 1.3. A monoclonal antibody IHC map

Immunohistochemicalof

Paraffin-embedded humanprostate cancerusingmonocolonalantibodyatdilutionof1:100

2. High sensitivity antibody customization

Our company has successfully developed a high-sensitivity antibody for customers with sensitivity up to 1.56pg/ml, and ELISA verification can identify natural samples such as serum.

Figure 2.1. Standard curve of a monoclonal antibody

3. Small molecule and peptide antibody customization

Our company has successfully developed a high-sensitivity small molecule antibody for our customers. At present, our customers have successfully developed kits, and the linear range has reached the market requirements. A polypeptide preparation monoclonal antibody was identified by WB to identify a natural sample.

Figure 3.1. Validation of WB for monoclonal antibody preparation of a polypeptide